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Re: Let's do science! Experiment A: SPROUTING
The scientific method is a beautiful thing. I consider experimental design and statistical analysis to be fascinating. I am loving this thread... enjoying the science and seeing how we interact with it.
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The Rickeybird - 33 year old Patagonian Conure
The Scrapbook. 1984-?. http://www.parrotforums.com/incredib...4-updates.html The Star. Cinema verite! https://www.youtube.com/channel/UCyC...22fVRRwfLk9gwA The Artist. He chews holes -uh- designs - in cloth. I sell them in my eBay Gallery. The Pet of the Day. Suggest one!http://petoftheday.com/archive/2016/May/20.html ![]() |
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Re: Let's do science! Experiment A: SPROUTING
Kentuckienne, I am a biologist by training, so I have experience in biological experimental design.
Not to belabor the point (understand I'm not at all trying to be nasty or come at you, just adding the actual biological experience here), but my contribution here is to temper your expectations - not necessarily dissuade you from doing it. You aren't able to correctly identify the bacteria you are culturing - last checked you don't have a several thousand dollar DNA sequencer. If you can't even identify what you are culturing you cannot draw any valid conclusions. Microbiology is fickle that way. As you somewhat implied yourself when you acknowledged no biological experience above, biology is not chemistry. |
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Re: Let's do science! Experiment A: SPROUTING
I found that news stuff I was talking about.
High bacteria levels in bean sprouts: CBC probe - Manitoba - CBC News Growing Sprouts in Retail Food Establishment - CFP Issues 02-III-01 and 04-III-012 Not safe to eat: Three foods to avoid - CNN.com and about home-growing... https://www.foodsafety.gov/keep/type...s/sprouts.html Fortunately, we don't LIKE them, so it's no skin off our proverbial buttfeathers.
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The Rickeybird - 33 year old Patagonian Conure
The Scrapbook. 1984-?. http://www.parrotforums.com/incredib...4-updates.html The Star. Cinema verite! https://www.youtube.com/channel/UCyC...22fVRRwfLk9gwA The Artist. He chews holes -uh- designs - in cloth. I sell them in my eBay Gallery. The Pet of the Day. Suggest one!http://petoftheday.com/archive/2016/May/20.html ![]() |
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Re: Let's do science! Experiment A: SPROUTING
Kentuckienne, I am a biologist by training, so I have experience in biological experimental design.
Not to belabor the point (understand I'm not at all trying to be nasty or come at you, just adding the actual biological experience here), but my contribution here is to temper your expectations - not necessarily dissuade you from doing it. You aren't able to correctly identify the bacteria you are culturing - last checked you don't have a several thousand dollar DNA sequencer. If you can't even identify what you are culturing you cannot draw any valid conclusions. Microbiology is fickle that way. As you somewhat implied yourself when you acknowledged no biological experience above, biology is not chemistry. Coliscan EasyGel, test kit for 10 tests is about $30, uses pre-treated dishes and media that inhibits other bacteria to quantify (I assume roughly) E. Coli: https://www.micrologylabs.com/page/93/Coliscan-Easygel General presence/absence tests for coliform and e.coli, simply indicate present or not, without quantifying how much is in each sample. These are fairly inexpensive and available from lots of places, such as "Industrial Test Systems 487197 WaterWorks EZ Cult Bacteria Test" from Amazon Interesting and simple semi-quantitative test from Aquagenx, haven't found the price yet: https://www.aquagenx.com/wp-content/...er_Sobsey1.pdf So far, Coliscan EasyGel looks like the best option, but I'll keep looking. The CDC says: Reduce your risk of getting sick from raw sprouts. Sprouts are a known source of foodborne illness. Children, older adults, pregnant women, and people with weakened immune systems should avoid eating raw sprouts of any kind (including alfalfa, clover, radish, and mung bean sprouts). Cook sprouts thoroughly to reduce the risk of illness. Cooking sprouts thoroughly kills the harmful bacteria. Request that raw sprouts not be added to your food. If you purchase a sandwich or salad at a restaurant or deli, check to make sure that raw sprouts have not been added. |
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Re: Let's do science! Experiment A: SPROUTING
I found that news stuff I was talking about.
High bacteria levels in bean sprouts: CBC probe - Manitoba - CBC News Growing Sprouts in Retail Food Establishment - CFP Issues 02-III-01 and 04-III-012 Not safe to eat: Three foods to avoid - CNN.com and about home-growing... https://www.foodsafety.gov/keep/type...s/sprouts.html Fortunately, we don't LIKE them, so it's no skin off our proverbial buttfeathers. ![]() Edit: Digging deeper myself I have found this https://www.cdc.gov/salmonella/readi...16/advice.html This relates to 2016 so is worth reading IMO. So in the interests of safety all those in the US that buy their sprouts for their own consumption or their birds need to exercise caution. I am happy with the source of sprouts I am able to purchase in the UK/EU.
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Last edited by plumsmum2005; 01-05-2017 at 04:19 AM. |
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Re: Let's do science! Experiment A: SPROUTING
Hey, what's the worst that can happen, I learn nothing. More likely I'll learn something on a macro scale that provides some useful information. I probably learn more from doing literature searches and relying on trusted sources, but it's so much FUN to play with science! Just the mental effort to design an experiment, carry it out and interpret the results is worthwhile even if the results aren't useful. It's good thinking practice. That's why I opened the project to everyone who wants to be involvement or have input. I sure don't know what I'm doing, but that's never stopped me yet!
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SassiBird (01-06-2017) |
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Re: Let's do science! Experiment A: SPROUTING
Hey, what's the worst that can happen, I learn nothing. More likely I'll learn something on a macro scale that provides some useful information. I probably learn more from doing literature searches and relying on trusted sources, but it's so much FUN to play with science! Just the mental effort to design an experiment, carry it out and interpret the results is worthwhile even if the results aren't useful. It's good thinking practice. That's why I opened the project to everyone who wants to be involvement or have input. I sure don't know what I'm doing, but that's never stopped me yet!
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